Fig. 2

Macroscopical and histopathological findings in HCC with OGCs. Macroscopically, the tumor section appears solid with local evidence of bleeding, necrosis, and a gray-white, hard texture (A). The tumor is composed of two components: pleomorphic mononuclear cells and osteoclast-like giant cells (B, C) with intravascular tumor emboli (D). Immunohistochemical staining shows positive expression of cytokeratin (AE-1/AE-3) in mononuclear cells and negative expression in OGCs (E); tissue macrophage marker (CD68) is negative in the mononuclear cell area but strongly positive in the OGCs area (F). H&E staining: A, B, D × 100, C × 200. Immunohistochemical staining: E, F × 200