Table 2 The genetically engineered mouse models of hepatocellular carcinoma
From: An overview of mouse models of hepatocellular carcinoma
Author | Model | Technology | Mouse strains | Method |
|---|---|---|---|---|
Xue et al. [43] | HCC | Sleeping beauty CRISPR-Cas9 system | FVB | Hydrodynamic injection of a CRISPR plasmid DNA expressing Cas9 and sgRNAs to the liver and directly target the tumor suppressor genes Pten and p53, alone and in combination. |
Bell et al. [44] | HCC | Sleeping beauty transposon system | C57BL/6 | The rapid, high-pressure injection of a naked plasmid DNA solution (2ml DNA/20 g mouse) into the tail vein within a period of 4 − 7 s. |
Katz et al. [47] | HCC | Cre-loxp | C57BL/6 | Conditional Trp53F2–10/F2–10 KO mice were crossed with AFP-cre mice. |
Calvisi et al. [48] | HCC | Cre-loxp | C57 | TG mice were generated by crossing homozygous c-Myc with homozygous E2F1 mice. |
Tward et al. [49] | HCC | Sleeping beauty transposon system | FVB/N | Ten to 50 micrograms of the plasmids encoding the Sleeping Beauty transposase and transposons with oncogenes of interest in a ratio of 1:25 were diluted in 2.5 ml of filtered 0.9% NaCl and then injected into the lateral tail veins of 6- to 8-week-old mice. |
Cao et al. [50] | HCC | CRISPR-Cas9 and sleeping beauty transposon system | C57BL/6 | Plasmids pCMV/SB, PT3-EF1a-c-Met, PT3-△90-β-catenin, Lenti CRISPR-sgPten and LentiCRISPR-sgp53 were injected into mice at a dosage of 10 µg/mouse within 3–5 s/time. |
Ochiai et al. [51] | HCC | Cre-loxp | C57BL/6 | Conditional TFF1 KO mice were crossed with Alb-cre mice to generate a TFF1-deficient HCC mouse model (KC/TFF1−/−). |
Kenerson et al. [53] | HCC | Cre-loxp | C57BL/6 | Tsc1f/f and Ptenf/f mice were separately bred with Alb-cre mice to generate Tsc1f/f; Alb-cre and Ptenf/f; Alb-cre mice respectively. Double knockout mutant mice (Tsc1f/f; Ptenf/f;Alb-cre) were created by crossing Tsc1f/f;Alb-cre with Ptenf/f; Alb-cre mice to generate Tsc1f/+;Ptenf/+;Alb-cre mice. |
Sekine et al. [54] | HCC | Cre-loxp | C57 | Conditional Ctnnb1 KO mice were crossed with Alb-cre mice to generate an Alb-cre; Ctnnb1f/f mouse model, which developed hepatocellular adenomas and carcinomas at age of 1 year. |
Zheng et al. [55] | HCC | Cre-loxp | C57BL/6 | We crossed CAG-loxp-LacZ T antigen mice with Alb-cre mice, and observed HCC at 3–10 months of age. |
Ruiz et al. [58] | HCC | CRISPR-Cas9 system | C57BL/6 | Hydrodynamic injection of px330-sg-p53 and CMV-SB13 in combination with MYC-luc or MYC-lucOS into 6-week-old female mice. The majority of MYC-luc; sg-p53 mice presented gross liver tumors that caused death with a median survival of 35–44 days. |