Fig. 4

GP73 activates CSC-related genes in L02 and HepG2 cells. A–C Percentage changes in CD133⁺ (A), CD117⁺ (B), and CD90⁺ (C) cells in the L02 and HepG2 GP73-overexpressing cells were measured by flow cytometry. L02-GFP and HepG2-GFP cells were employed as controls compared with L02-GP73 and HepG2-GP73 cells. D, E The mRNA levels of Klf4, Sox2, Nanog, c-Myc, and Oct4 in the L02 and HepG2 GP73-overexpressing cells were determined by qRT-PCR. The graphs show the means ± SDs, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the control group