Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor

Abdul-Wahab, Osama Mohammed Saed; Al-Shyarba, Mishari H.; Mardassi, Boutheina Ben Abdelmoumen; Sassi, Nessrine; Al Fayi, Majed Saad Shaya; Otifi, Hassan; Al Murea, Abdullah Hassan; Mlik, Béhija; Yacoub, Elhem

doi:10.1186/s13027-021-00344-9

Infectious Agents and Cancer

Table 1 Characteristics of primers used for detection of mollicutes investigated in this study

From: Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor

Mycoplasma species	Primer designation	Target gene	Gene locus	Product	Amplicon size (bp)	Sequence (5′-3′)	References
M. hominis	Mhp120’F Mhp120’R	p120’	MHO_RS02125	DUF885 family protein	190	GTTTTAGAACGTAAAATTCTA CATCAGCAGGATTGTTTAAAGCT	This study^a
M. hominis	Mh16SF Mh16SR	rrnA16S	MHO_RS01540	16S ribosomal RNA	164	GGTAATGGCCCACCAAGACTAT GAAGACCTTCATCGTGCACGCTG	This study^a
M. genitalium	MgPa335F MgPa432R	mgpA	MG_RS01075	Adhesin protein	78	GAGAAATACCTTGATGGTCAGCAA GTTAATATCATATAAAGCTCTACCGTTGTTATC	[43]
M. genitalium	Mg16SF MG16SR	rrnA16S	MG_RS00775	16S ribosomal RNA	195	TAGCTAATACCGCATAAGAACTTT CTCCCGTAGGAGTATGGGCCGTGT	This study^a
M. fermentans	RW 005 RW 004	IS1630I	MBIO_RS03870	IS30-like element	205	GGTTATTCGATTTCTAAATCGCCT GGACTATTGTCTAAACAATTTCCC	[44]
M. fermentans	Mf16SF Mf16SR	rrnA16S	MBIO_RS4090	16S ribosomal RNA	186	TTGACGGTACCTTATTAGAAA TCTACACAACTCTAGCCTG	This study^a
U. urealyticum	UUureF UUureR	ureB	UUR8_RS02385	Urease subunit beta	100	GATCACATTTCCACTTATTTGAAACA AAACGACGTCCATAAGCAACTTTA	[45]
U. urealyticum	Uu16SF Uu16SR	rrnA16S	UUR8_RS00640	16S ribosomal RNA	181	TGCCTGGGTAGTACATTCGCAAGAA GACAACCATGCACCACCTGTC	This study^a
U. parvum	UPureF UPureF	ureB	UPA3_RS02275	Urease subunit beta	99	GATCACATTTTCACTTGTTTG AAGTG AACGTCGTCCATAAGCAACTTTG	[45]
U. parvum	Up16SF Up16SF	rrnA16S	UPA3_RS00595	16S ribosomal RNA	183	CTTTTATATGGGAAGAAACGCT GCATCTAGATTTAATACC	This study^a
Human DNA	HmitoDNAF HmitoDNAR	MT-CO3	YP_003024032.1	Cytochrome c oxidase subunit III	208	GGATAATCCTATTTATTACCTCAGAAG AGACTATGGTGAGCTCAGGTGATTGAT	This study^a

^aPrimers for each gene were designed based on sequences available in GenBank

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ISSN: 1750-9378

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