Association between oncogenic human papillomavirus type 16 and Killian polyp

Oton-Gonzalez, Lucia; Rotondo, John Charles; Cerritelli, Luca; Malagutti, Nicola; Lanzillotti, Carmen; Bononi, Ilaria; Ciorba, Andrea; Bianchini, Chiara; Mazziotta, Chiara; De Mattei, Monica; Pelucchi, Stefano; Tognon, Mauro; Martini, Fernanda

doi:10.1186/s13027-020-00342-3

Infectious Agents and Cancer

Table 1 Primers used in qPCR to detect and quantify HPV, PyV DNA, viral, cellular genes

From: Association between oncogenic human papillomavirus type 16 and Killian polyp

	Target	Primers names	Primers sequence (5′→ 3′)	Amplicon size (bp)	Annealing temp. (°C)	References
DNA
Viral	HPV L1	GP5+	TTTGTTACTGTGGTAGATACTAC	139–145	48	Malagutti et al. 2020 [33]; Tognon et al. 2020 [34]; Rotondo et al. 2020a [35]
		GP6+	GAAAAATAAACTGTAAATCATATTC
	HPV16 E2	E- HPV16 E2 F	AACGAAGTATCCTCTCCTGAAATTATTAG	82	60	Peitsaro, Johansson, e Syrjänen 2002 [37]
		E- HPV16 E2 R	CCAAGGCGACGGCTTTG
		E Probe 16E2PRO	[ROX] CACCCCGCCGCGACCCATA [BHQ2]
	HPV16 E6	I+E- HPV16 E6 F	GAGAACTGCAATGTTTCAGGACC	81	60
		I+E- HPV16 E6 R	TGTATAGTTGTTTGCAGCTCTGTGC
		I+E Probe 16E6PRO	[6FAM] CAGGAGCGACCCAGAAAGTTACCACAGTT [BHQ1]
	MCPyV	RQ MCPyV_LT.1F	CCACAGCCAGAGCTCTTCCT			Tagliapietra et al. 2020 [38]
		RQ MCPyV_LT.1R	TGGTGGTCTCCTCTCTGCTACTG
		RQ MCPyV_LT Probe	[6FAM] TCCTTCTCAGCGTCCCAGGCTTCA [MGB]
	JCPyV	Assay_JCyV	AI1RWNE			Tagliapietra et al. 2019 [39]
	BKPyV	Assay_BKyV	AI20UTM
Host	β-Globin	β-Globin F	TGGGTTTCTGATAGGCACTGACT	152	56	Contini et al. 2018 [32]
Host		β-Globin R	AACAGCATCAGGAGTGGACAGAT
RNA
Viral	HPV16 E2	HPV16 E2 F	AACGAAGTATCCTCTCCTGAAATTATTAG	82	60	Peitsaro, Johansson, e Syrjänen 2002 [37]
		HPV16 E2 R	CCAAGGCGACGGCTTTG
	HPV16 E6	HPV16 E6 F	GAGAACTGCAATGTTTCAGGACC	81	60
		HPV16 E6 R	TGTATAGTTGTTTGCAGCTCTGTGC
	HPV16 E5	16-E5 FWD	CGTCCGCTGCTTTTGTCTGTGTCTACATAC	89	60	Weyn et al. 2011 [40]
		16-E5 REV	CACCTAAACGCAGAGGCTGCTGTTATCCAC
	HPV16 E7	E7 FWD	AGGAGGATGAAATAGATGGTCCAG	112	60	Pett et al. 2006 [41]
		E7 REV	CTTTGTACGCACAACCGAAGC
Host	P16INK4A	p16 ink4a FWD	CCAACGCACCGAATAGTTACG	58	60	Marcoux et al. 2013 [42]
		p16 ink4a REV	GCGCTGCCCATCATCATG
	GAPDH	GAPDH F	GAAGGTGAAGGTCGGAGTC	226	60	Xiao et al. 2011 [43]
		GAPDH R	GAAGATGGTGATGGGATTTC

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