Fig. 2

Macrophage polarization and PD-L1 expression on TAMs: (a) CD68 (brown), CD163 (red), c-maf (blue). The majority of TAMs express M2 phenotype markers (CD163+, c-maf+). (O.M: 10x). Inset: CD8 (red), Granzyme B (blue) and PD-1(brown), pattern of PD1 expression on cytotoxic T cells. (b) C-MYC (blue),PD-L1 (brown), CD163 (red); the majority of TAMs in eBL and idBL cases expressed PD-L1, in addition triple staining disclosed clusters of C-MYC/PD-L1 double positive cells in 2 cases characterized by co-expression of LMP2A (Inset double IF) clustering with TAMs (O.M: 40x)